Ralf Stumm

Otto von Guericke University of Magdebur, Germany

Abstract

[O13] Regulation of adult hippocampal neurogenesis by the SDF-1-chemokine receptor CXCR4

Stromal-cell-derived factor-1 (SDF-1) and its receptor CXC chemokine receptor 4 (CXCR4) play a well-established role during embryonic development of dentate gyrus granule cells. However, little is known about the regulation and function of CXCR4 in the postnatal dentate gyrus. Here, we identify a striking mismatch between intense CXCR4 mRNA and limited CXCR4 protein expression in adult rat subgranular layer (SGL) neurons. We demonstrate that CXCR4 protein expression in SGL neurons is progressively lost during postnatal day 15 (P15) to P21. This loss of CXCR4 protein expression was paralleled by a reduction in the number of SDF-1-responsive SGL neurons and a massive upregulation of SDF-1 mRNA in granule cells. Intraventricular infusion of the CXCR4-antagonist AMD3100 dramatically increased CXCR4 protein expression in SGL neurons, suggesting that CXCR4 is tonically activated and downregulated by endogenous SDF-1. Infusion of AMD3100 also facilitated detection of CXCR4 protein in bromodeoxyuridine-, nestin-, and doublecortin-labeled cells and showed that the vast majority of adult-born granule cells transiently expressed CXCR4. Chronic AMD3100 administration impaired formation of new granule cells as well as neurogenesis-dependent long-term recognition of novel objects. Therefore, our findings suggest that tonic activation of CXCR4 in newly formed granule cells by endogenous SDF-1 is essential for neurogenesis-dependent long-term memory in the adult hippocampus.

Key words: neurogenesis, CXCL12, SDF-1, CXCR4

Biography

Ralf Stumm is Head of the Research Group Molecular and Systemic Neuropharmacology at the Center for Behavioral Brain Science Magdeburg, Germany. He obtained his Ph.D. in Molecular Medicine at the Institute of Anatomy and Cell Biology, Philipps-University Marburg, Germany. His current research employs histochemical, biochemical, genetic, and behavioral approaches to investigate the function of neuropeptides and chemokines in the brain.